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Description
Porcine ET-3 (Endothelin 3) ELISA KitThe Porcine (ET 3) Endothelin 3 ELISA Kit measures Endothelin 3 in Porcine samples. The plate has been pre coated with Porcine ET 3 antibody. ET 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine ET 3 Antibody is added and binds to ET 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 3 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Porcine (ET-3) Endothelin 3 ELISA Kit measures Endothelin 3 in Porcine samples. The plate has been pre-coated with Porcine ET-3 antibody. ET-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine ET-3 Antibody is added and binds to ET-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine ET-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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